Format

Send to:

Choose Destination
See comment in PubMed Commons below
Exp Eye Res. 2014 Mar;120:50-4. doi: 10.1016/j.exer.2013.12.006. Epub 2013 Dec 15.

High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels.

Author information

  • 1Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy. Electronic address: pvnbbr@unife.it.
  • 2Dept of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.

Abstract

Human retinal pigment epithelium cells were used to investigate the mechanisms underlying blood-retinal barrier disruption under conditions of chronic hyperglycemia. The treatment with 25 mM glucose caused a rapid drop in the transepithelial electrical resistance (TEER), which was reversed by the addition of either a methanolic extract from Goji (Lycium barbarum L.) berries or its main component, taurine. Intracellular cAMP levels increased concurrently with the high glucose-induced TEER decrease, and were correlated to an increased activity of the cytosolic isoform of the enzyme adenylyl cyclase. The treatment with plant extract or taurine restored control levels. Data are discussed in view of a possible prevention approach for diabetic retinopathy.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

HRPE cells; TEER; adenylyl cyclase; high glucose; plant extract

PMID:
24345371
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk