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Anal Chem. 2014 Jan 21;86(2):987-92. doi: 10.1021/ac403261s. Epub 2013 Dec 27.

Biomolecular analysis and biological tissue diagnostics by electrospray ionization with a metal wire inserted gel-loading tip.

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  • 1Clean Energy Research Center, University of Yamanashi , 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.


A metal wire-inserted disposable gel-loading tip was examined as an electrospray emitter. Its performance was similar to that of conventional electrospray ionization (ESI) with a relatively low flow rate (~100 nL/min) and without the need for solvent pumps. It was also used as an emitter for solid probe-assisted ESI (SPA-ESI) (e.g., biofluid was sampled from the biological tissue by a needle and was inserted into the solvent-preloaded gel-loading tip). Selective detection of lipids and proteins, such α and β chains of hemoglobin could be accomplished by choosing appropriate solvents. A suitable protocol for cancer diagnosis was established by this method. A good figure of merit of this method is its applicability to biological tissue diagnostics with high cost efficiency and on a disposable basis.

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