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Cancer Lett. 2014 Apr 1;345(1):65-74. doi: 10.1016/j.canlet.2013.11.009. Epub 2013 Dec 11.

Circadian properties of cancer stem cells in glioma cell cultures and tumorspheres.

Author information

  • 1Department of Biological Sciences and the JP Scott Center for Neuroscience, Mind, and Behavior, Bowling Green State University, Bowling Green, OH 43403, United States.
  • 2Department of Biological Sciences and the JP Scott Center for Neuroscience, Mind, and Behavior, Bowling Green State University, Bowling Green, OH 43403, United States. Electronic address: mgeusz@bgsu.edu.

Abstract

Increased cancer risk is linked to disruption of circadian rhythms. Cancer stem cells (CSCs) are a known cause of cancer aggressiveness, but their circadian properties have not been described. We discovered circadian rhythms in gene expression within C6 glioma tumorspheres enriched in CSCs and found that the circadian clock is particularly robust in medium lacking any growth factors. A method is introduced for identifying individual CSCs in culture for single-cell analysis. CSCs in monolayer cell culture failed to show a circadian rhythm in nuclear localization of mPER2 protein, suggesting that cell interactions or the tumor-like microenvironment within tumorspheres enable circadian timing.

Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

KEYWORDS:

C6; Cancer stem cell; Circadian rhythms; Glioma; Tumorsphere

[PubMed - indexed for MEDLINE]
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