Format

Send to

Choose Destination
See comment in PubMed Commons below
J Pharm Biomed Anal. 2014 Mar;90:15-26. doi: 10.1016/j.jpba.2013.11.021. Epub 2013 Nov 27.

New triterpene saponins from the roots of Glycyrrhiza yunnanensis and their rapid screening by LC/MS/MS.

Author information

  • 1State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
  • 2Agilent Technologies, 3 Wangjing North Road, Beijing 100102, China.
  • 3State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: yemin@bjmu.edu.cn.

Abstract

The roots of Glycyrrhiza yunnanensis Cheng f. et L. K. Dai ex P. C. Li are used as licorice in traditional medicine of Southwest China. Triterpene saponins are the major chemical constituents. In this study, one new oleanane-type triterpenoid, glyyunnansapogenin I (I), seven new triterpene saponins, yunganosides E3 (II), L (III), M (IV), N1 (V), O (VI), P (VII) and N2 (VIII), together with four known saponins (IX-XII) were isolated from the roots of G. yunnanensis by preparative chromatography. Their structures were identified by spectroscopic analysis including NMR and HR-MS. Based on (-)-ESI-MS/MS fragmentation behaviors of these reference standards, an LC/MS/MS method using neutral loss scan and precursor ion scan on a triple quadrupole mass spectrometer was established to rapidly and comprehensively analyze triterpene saponins in G. yunnanensis. Combined with high-accuracy qTOF mass spectrometry analysis, a total of 50 saponins were detected, and their structures were identified or tentatively characterized. This is the first systematic study on triterpene saponins in G. yunnanensis.

Copyright © 2013. Published by Elsevier B.V.

KEYWORDS:

Glycyrrhiza yunnanensis; Glyyunnansapogenin I; LC/MS/MS; Triterpene saponin; Yunganoside

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk