Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15;945-946:39-45. doi: 10.1016/j.jchromb.2013.11.036. Epub 2013 Nov 25.

Efficient conversion of myricetin from Ampelopsis grossedentata extracts and its purification by MIP-SPE.

Author information

  • 1College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
  • 2College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Electronic address: yanwangcsu@163.com.

Abstract

In this study, we developed an efficient conversion process of dihydromyricetin to myricetin from Ampelopsis grossedentata extracts. The content of myricetin increased from 2.38% to 85.57%, demonstrating the successful dehydrogenation of dihydromyricetin. Molecularly imprinted polymers (MIPs) were prepared by surface imprinting method using silica microspheres as the support matrices and myricetin as template. The MIPs were applied for the selective adsorption of myricetin. The chemical structure of the MIPs was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Static, dynamic and selective adsorption experiments showed that the MIPs exhibited good adsorption ability, rather fast template rebinding kinetics, and appreciate selectivity over structurally related compounds. Accordingly, the MIPs were applied as the selective sorbent in SPE to purify myricetin obtained through dehydrogenation, followed by HPLC-UV analysis. The recoveries of myricetin and dihydromyricetin were 92.7% and 55.6%, respectively. This study demonstrates the feasibility of using the developed MIP-SPE method to purify and enrich myricetin in the natural products.

Copyright © 2013 Elsevier B.V. All rights reserved.

KEYWORDS:

Ampelopsis grossedentata; Dehydrogenation; Molecularly imprinted microspheres; Myricetin; SPE

PMID:
24321759
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk