Display Settings:

Format

Send to:

Choose Destination
Genet Mol Res. 2013 Nov 22;12(4):5911-5. doi: 10.4238/2013.November.22.19.

Isolation and characterization of polymorphic microsatellite loci in the swimming crab Portunus trituberculatus (Portunidae).

Author information

  • 1Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, China.

Abstract

The Swimming crab Portunus trituberculatus (Portunidae) is an important economically food species. To provide molecular markers for P. trituberculatus, we isolated and characterized polymorphic microsatellite markers. We developed a 5'-anchored genomic library of P. trituberculatus DNA, and derived 45 positive clones. We designed 30 pairs of primers from the sequences of these clones, and 10 of which were polymorphic. The loci were screened in 31 P. trituberculatus individuals; the number of alleles ranged from 2 to 5. The observed heterozygosity ranged from 0.20 to 0.49 and the expected heterozygosity ranged from 0.50 to 0.78. The polymorphism information content per locus ranged from 0.37 to 0.75. These polymorphic microsatellite markers may provide some useful information for the population genetic studies of swimming crab and other Portunus species.

PMID:
24301961
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Fundacao de Pesquisas Cientificas de Ribeirao Preto
    Loading ...
    Write to the Help Desk