Pedopeptins, novel inhibitors of LPS: taxonomy of producing organism, fermentation, isolation, physicochemical properties and structural elucidation

Yuki Hirota-Takahata; Shiho Kozuma; Nahoki Kuraya; Daisuke Fukuda; Mutsuo Nakajima; Osamu Ando

doi:10.1038/ja.2013.122

Pedopeptins, novel inhibitors of LPS: taxonomy of producing organism, fermentation, isolation, physicochemical properties and structural elucidation

J Antibiot (Tokyo). 2014 Mar;67(3):243-51. doi: 10.1038/ja.2013.122. Epub 2013 Dec 4.

Authors

Yuki Hirota-Takahata¹, Shiho Kozuma², Nahoki Kuraya³, Daisuke Fukuda¹, Mutsuo Nakajima³, Osamu Ando⁴

Affiliations

¹ Discovery Science and Technology Department, Daiichi-Sankyo RD Novare Co., Ltd., Tokyo, Japan.
² Biologics Research Laboratories, Daiichi-Sankyo Co., Ltd., Tokyo, Japan.
³ Exploratory Laboratories, Daiichi-Sankyo Co., Ltd., Tokyo, Japan.
⁴ Oncology Research Laboratories, Daiichi-Sankyo Co., Ltd., Tokyo, Japan.

PMID: 24301185
DOI: 10.1038/ja.2013.122

Abstract

In the course of our screening for inhibitors of lipopolysaccharide (LPS) binding to cellular receptor CD14, potent inhibitory activity was detected in the cultured broth of Pedobacter sp. SANK 72003. Three active compounds, pedopeptin A, B and C, were isolated from the broth and their structures were elucidated by physicochemical and spectral data to be new cyclic depsipeptides.

MeSH terms

Culture Media
Depsipeptides / chemistry
Depsipeptides / isolation & purification*
Fermentation
Lipopolysaccharide Receptors / metabolism
Lipopolysaccharides / antagonists & inhibitors
Lipopolysaccharides / metabolism
Pedobacter / chemistry*
Peptides, Cyclic / chemistry
Peptides, Cyclic / isolation & purification*
Spectrum Analysis

Substances

Culture Media
Depsipeptides
Lipopolysaccharide Receptors
Lipopolysaccharides
Peptides, Cyclic
pedopeptin A
pedopeptin B
pedopeptin C