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Nutrition. 2014 Mar;30(3):319-25. doi: 10.1016/j.nut.2013.08.003. Epub 2013 Dec 2.

Effect of yellow capsicum extract on proliferation and differentiation of 3T3-L1 preadipocytes.

Author information

  • 1Department of Stomatology, Children's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • 2College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
  • 3Department of Food Science & Nutrition, College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China.
  • 4Department of Food Science & Nutrition, College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, China. Electronic address: shrshen@zju.edu.cn.
  • 5UND Life Sciences, Shaker Heights, Ohio, USA; Jawaharlal Nehru Technological University, Kakinada, India; Bio-Science Research Centre, Gayatri Vidya Parishad College of Engineering, Visakhapatnam, India. Electronic address: undurti@hotmail.com.

Abstract

OBJECTIVES:

To evaluate the effect of effect of Yellow Capsicum extract (YCE) that is rich in capsaicin on the proliferation and differentiation of 3T3-L1 preadipocytes in vitro.

METHODS:

3T3 L1 cells that were exposed to differentiation-inducing medium containing high glucose DMEM (Dulbecco's Modified Eagle's Medium) and subsequently were treated with capsaicin and YCE for their effect on adipocyte differentiation, changes in their triglyceride content, leptin secretion, expression of lipoprotein lipase, PPARγ, and CCAAT/enhancer-binding protein alpha (C/EBPα).

RESULTS:

Both YCE and capsaicin inhibited proliferation and differentiation 3T3-L1 preadipocytes and suppressed accumulation of intracellular triglyceride in a dose-dependent manner. In addition, a significant decrease in the expression of lipoprotein lipase (LPL), leptin, PPARγ, and C/EBPα was noted in 3T3-L1 preadipocytes when induced to differentiate by YCE and Capsaicin.

CONCLUSIONS:

The potent inhibitory action of YCE and Capsaicin on the differentiation of 3T3-L1 preadipocyte observed suggests that they (YCE and Capsaicin) have the potential to inhibit obesity that needs to be explored in future studies.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

3T3-L1; Differentiation; Obesity; Proliferation; Yellow capsicum extract

PMID:
24296036
[PubMed - indexed for MEDLINE]
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