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Fish Shellfish Immunol. 2014 Jan;36(1):240-51. doi: 10.1016/j.fsi.2013.11.009. Epub 2013 Nov 26.

Immune gene discovery in the crucian carp Carassius auratus.

Author information

  • 1Research Institute for Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
  • 2Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
  • 3Department of Life Science, College of Convergence, Sangmyung University, Seoul 110-743, South Korea.
  • 4Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840, South Korea.
  • 5Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, South Korea.
  • 6Research Strategy & Planning Division, National Institute of Environmental Research, Incheon 404-708, South Korea. Electronic address: leecwoo@korea.kr.
  • 7Department of Biological Sciences, College of Natural Sciences, Sungkyunkwan University, Suwon 440-746, South Korea. Electronic address: r_marmoratus@hanmail.net.

Abstract

The crucian carp Carassius auratus (Cyprinidae) is one of the important fish species in aquaculture. Although the crucian carp has several economic benefits, their immune system and gene information have not been investigated in depth as yet. Here, we performed the transcriptome analysis of C. auratus using the pyrosequencing method and selected several immune-related genes. Of unigenes obtained in this species, we identified a number of immune system-related genes (e.g. adhesive protein, antimicrobial protein, apoptosis- and cell cycle-related protein, cellular defense effector, immune regulator, pattern recognition protein, protease, protease inhibitor, reduction/oxidation-related protein, signal transduction-related protein and stress protein) that are potentially useful for studies on fish immunity. To be of public and practical use, we designed primer pairs of each gene from the crucian carp for real-time RT-PCR application and tested the amplicon identity of entire gene sets with the total RNA sample. For comparative analysis, we measured tissue-preferential transcript profiles of selected genes. This study will be helpful to extend our knowledge on the immune system of the crucian carp in comparative aspects and to develop the crucian carp as a potential model organism for aquatic quality monitoring in fish farming.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

Carassius auratus; Crucian carp; Immune-relevant genes; Pyrosequencing

PMID:
24287371
[PubMed - indexed for MEDLINE]

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