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Chemistry. 2013 Dec 2;19(49):16625-33. doi: 10.1002/chem.201302874. Epub 2013 Nov 6.

A self-assembled nanohybrid composed of fluorophore-phenylamine nanorods and Ag nanocrystals: energy transfer, wavelength shift of fluorescence and TPEF applications for live-cell imaging.

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  • 1Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279.


A fluorophore-phenylamine derivative (L) has been coupled with silver nanocrystals (NCs) to construct an L-Ag nanohybrid. Owing to synergic effects of the L and Ag components, the exciton-plasmon interactions between L and Ag increase the strength of the donor-acceptor interaction within the nanohybrid, a fact that results in an energy-transfer process and further brings about a dramatic redshift of single-photon absorption and fluorescence, and a decreased fluorescence FL lifetime. The coupling effect also leads to enhancement of a series of nonlinear optical properties, including two-photon-excited fluorescence (TPEF), two-photon-absorption (TPA) cross section (δ), two-photon-absorption coefficient (β), nonlinear refractive index (γ), and third order nonlinear optical susceptibility (χ((3))). The enhanced two-photon fluorescence of the nanohybrid is proven to be potentially useful for two-photon microscopy of live cells, such as HepG2. Moreover, cytotoxicity tests show that the low-micromolar concentrations of the nanohybrid do not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.

Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


TPEF cell imaging; dye-metal nanohybrid; energy transfer; exciton-plasmon interaction; nonlinear optics

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