Send to:

Choose Destination
See comment in PubMed Commons below
Acta Trop. 2014 Mar;131:41-6. doi: 10.1016/j.actatropica.2013.11.010. Epub 2013 Nov 22.

New insights about cross-reactive epitopes of six trypanosomatid genera revealed that Crithidia and Leptomonas have antigenic similarity to L. (L.) chagasi.

Author information

  • 1Instituto de Medicina Tropical de São Paulo da Universidade de São Paulo (USP), São Paulo, SP, Brazil.
  • 2Departamento de Parasitologia do Instituto de Ciências Biomédicas-USP, São Paulo, SP, Brazil.
  • 3Departamento de Patologia da Faculdade de Medicina-USP, São Paulo, SP, Brazil.
  • 4Universidade Federal de São Paulo, São Paulo, SP, Brazil.
  • 5Instituto de Infectologia Emílio Ribas de São Paulo, São Paulo, SP, Brazil.
  • 6Instituto de Medicina Tropical de São Paulo da Universidade de São Paulo (USP), São Paulo, SP, Brazil; Departamento de Medicina Preventiva da Faculdade de Medicina- USP, São Paulo, SP, Brazil. Electronic address:


We investigated whether ELISA using crude antigens from insect and plant trypanosomatids, which are non-pathogenic and easily cultivated in large scale, has the same positivity data as Leishmania (Leishmania) chagasi, the etiological agent of human visceral leishmaniasis (VL) or canine leishmaniasis (CanL), or as Trypanosoma cruzi, the etiological agent of Chagas disease (CD). The antigens from Crithidia fasciculata, Crithidia luciliae, and Leptomonas seymouri showed 100% cross-reactivity with VL and CanL samples, with no statistically titers differences from L. (L.) chagasi, however, 34% (17/50) of VL samples revealed higher titers using the insect trypanosomatids than the homologous antigen. On the other hand, antigens from Strigomonas culicis, Angomonas deanei, and Phytomonas serpens showed low cross-reactivity with VL and CanL samples. The sera from patients with American tegumentary leishmaniasis showed low levels of cross-reactivity with all trypanosomatids investigated, even with L. (L) chagasi, without titers dissimilarity among them. These parasites were also worthless as antigen source for detection of CD cases, which required homologous antigens to reach 100% positivity. This study showed, by ELISA, that crude extract of Crithidia and Leptomonas have epitopes similar to L. (L.) chagasi, which supports the idea of using them as antigens source for the serodiagnosis of visceral leishmaniasis.

Published by Elsevier B.V.


Crithidia; Cross-reactivity; L. (L.) chagasi; Leptomonas; Trypanosoma cruzi; Trypanosomatids

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk