The rearrangement of filamentous actin in mossy fiber synapses in pentylenetetrazol-kindled C57BL/6 mice

Yan-Feng Zhang; Shu-Lei Li; Tian-Qing Xiong; Li-Bin Yang; Yong-Nan Li; Bai-Hong Tan; Qun Liu; Yan-Chao Li

doi:10.1016/j.eplepsyres.2013.10.019

The rearrangement of filamentous actin in mossy fiber synapses in pentylenetetrazol-kindled C57BL/6 mice

Epilepsy Res. 2014 Jan;108(1):20-8. doi: 10.1016/j.eplepsyres.2013.10.019. Epub 2013 Nov 11.

Authors

Yan-Feng Zhang¹, Shu-Lei Li², Tian-Qing Xiong², Li-Bin Yang³, Yong-Nan Li⁴, Bai-Hong Tan⁵, Qun Liu⁶, Yan-Chao Li⁷

Affiliations

¹ Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China; Department of Neurology, First Affiliated Hospital of Jilin University, Changchun, Jilin Province 130021, PR China; Department of Pediatric Neurology, First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
² Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China.
³ Department of Pediatric Neurology, First Hospital of Jilin University, Changchun, Jilin Province 130021, PR China.
⁴ Department of Neurology, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, PR China.
⁵ Laboratory Teaching Center of Basic Medicine, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China.
⁶ Department of Neurology, First Affiliated Hospital of Jilin University, Changchun, Jilin Province 130021, PR China. Electronic address: Liuqun7654321@126.com.
⁷ Department of Histology and Embryology, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University, Changchun, Jilin Province 130021, PR China. Electronic address: liyanchao@jlu.edu.cn.

PMID: 24275550
DOI: 10.1016/j.eplepsyres.2013.10.019

Abstract

Chemical kindling, as an experimental model of epileptogenesis, is induced by repetitive administration of subconvulsive amount of excitatory drugs. Kindled mice do not typically display spontaneous recurrent seizures, but are instead characterized by enhanced seizure susceptibility to convulsive stimulations. In order to provide insights into the aberrant synaptic plasticity during kindling, this study investigated the effect of pentylenetetrazol (PTZ) kindling on filamentous actin (F-actin) in mossy fiber synapses in C57BL/6 mice. Phalloidin labeling of F-actin showed that F-actin puncta were increased in number in the stratum lucidum of CA3 region in the hippocampus after kindling. The rearrangement of F-actin seemed to occur presynaptically, since synapsin I, a specific marker for mossy fiber terminals, was also up-regulated. Such subtle structural modifications occurring in the synapses are thought to contribute to the long-lasting increased sensitivity in the PTZ-kindled C57BL/6 mice.

Keywords: Chemical kindling; Filamentous actin; Hippocampus; Pentylenetetrazol; Synapsin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / drug effects
Actin Cytoskeleton / pathology*
Animals
CA3 Region, Hippocampal / drug effects
CA3 Region, Hippocampal / pathology
Kindling, Neurologic / drug effects
Kindling, Neurologic / pathology*
Male
Mice
Mice, Inbred C57BL
Mossy Fibers, Hippocampal / drug effects
Mossy Fibers, Hippocampal / pathology*
Pentylenetetrazole / toxicity*
Random Allocation
Seizures / chemically induced
Seizures / pathology*
Synapses / drug effects
Synapses / pathology*

Substances

Pentylenetetrazole