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Histopathology. 2014 Mar;64(4):547-56. doi: 10.1111/his.12278. Epub 2013 Nov 25.

BAI3, CDX2 and VIL1: a panel of three antibodies to distinguish small cell from large cell neuroendocrine lung carcinomas.

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  • 1Department of Pathology, Dow International Medical College, Karachi, Pakistan; Department of Pathology, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, UK; Divisions of Reproduction and Metabolic and Vascular Health, Warwick Medical School, Coventry, UK.

Abstract

AIMS:

Discriminating small-cell lung carcinoma (SCLC) from large-cell neuroendocrine carcinoma (LCNEC) rests on morphological criteria, and reproducibility has been shown to be poor. We aimed to identify immunohistochemical markers to assist this diagnosis.

METHODS AND RESULTS:

Gene expression profiling on laser captured frozen tumour samples from eight SCLC and eight LCNEC tumours identified a total of 888 differentially expressed genes (DEGs), 23 of which were validated by qRT-PCR. Antibodies to four selected gene products were then evaluated as immunohistochemical markers on a cohort of 173 formalin-fixed paraffin-embedded (FFPE) SCLC/LCNEC tumour samples, including 26 indeterminate tumours without a consensus diagnosis. Three markers, CDX2, VIL1 and BAI3, gave significantly different results in the two tumour types (P < 0.0001): CDX2 and VIL1 in combination (either marker positive) showed sensitivity and specificity of 81% for LCNEC while BAI3 showed 89% sensitivity and 75% specificity for SCLC. Of the 26 indeterminate tumours 15 (58%) showed an immunophenotype suggesting either SCLC or LCNEC, eight (31%) showed staining of both tumour types, and three (11%) were negative for all markers.

CONCLUSION:

A panel of three markers, BAI3, CDX2 and VIL1, is a useful adjunct in the diagnosis of these tumour types.

© 2013 John Wiley & Sons Ltd.

KEYWORDS:

BAI3; CDX2; VIL1; biomarkers; gene expression profiling; immunohistochemistry; large-cell neuroendocrine lung carcinoma; small-cell lung carcinoma

PMID:
24266897
[PubMed - indexed for MEDLINE]
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