Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies

B Thomas; S E Penn; G W Butcher; G Galfre

doi:10.1007/BF00393420

Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies

Planta. 1984 Mar;160(4):382-4. doi: 10.1007/BF00393420.

Authors

B Thomas¹, S E Penn, G W Butcher, G Galfre

Affiliation

¹ Plant Physiology Department, Glasshouse Crops Research Institute, Littlehampton, West Sussex, UK.

PMID: 24258587
DOI: 10.1007/BF00393420

Abstract

A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.