Format

Send to

Choose Destination
See comment in PubMed Commons below
Dev Biol. 2014 Feb 1;386(1):152-64. doi: 10.1016/j.ydbio.2013.11.012. Epub 2013 Nov 16.

Onset of atonal expression in Drosophila retinal progenitors involves redundant and synergistic contributions of Ey/Pax6 and So binding sites within two distant enhancers.

Author information

  • 1Department of Ophthalmology, Center for Vision Research, and SUNY Eye Institute, SUNY Upstate Medical University, Syracuse, NY, USA.
  • 2Ben May Institute for Cancer Research, Chicago University, Chicago, IL, USA.
  • 3Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • 4Department of Ophthalmology, Center for Vision Research, and SUNY Eye Institute, SUNY Upstate Medical University, Syracuse, NY, USA; Departments of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA; Department of Neuroscience & Physiology, SUNY Upstate Medical University, Syracuse, NY, USA. Electronic address: pignonif@upstate.edu.

Abstract

Proneural transcription factors drive the generation of specialized neurons during nervous system development, and their dynamic expression pattern is critical to their function. The activation of the proneural gene atonal (ato) in the Drosophila eye disc epithelium represents a critical step in the transition from retinal progenitor cell to developing photoreceptor neuron. We show here that the onset of ato transcription depends on two distant enhancers that function differently in subsets of retinal progenitor cells. A detailed analysis of the crosstalk between these enhancers identifies a critical role for three binding sites for the Retinal Determination factors Eyeless (Ey) and Sine oculis (So). We show how these sites interact to induce ato expression in distinct regions of the eye field and confirm them to be occupied by endogenous Ey and So proteins in vivo. Our study suggests that Ey and So operate differently through the same 3' cis-regulatory sites in distinct populations of retinal progenitors.

© 2013 Published by Elsevier Inc.

KEYWORDS:

Atoh7; Eye specification; Math5; Neurogenesis; Proneural factor

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk