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ACS Chem Biol. 2014 Feb 21;9(2):339-46. doi: 10.1021/cb400806r. Epub 2013 Nov 20.

A liquid array platform for the multiplexed analysis of synthetic molecule-protein interactions.

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  • 1Departments of Chemistry and Cancer Biology, The Scripps Research Institute, Scripps Florida , 130 Scripps Way, Jupiter , Florida 33458, United States.

Abstract

Synthetic molecule microarrays, consisting of many different compounds spotted onto a planar surface such as modified glass or cellulose, have proven to be useful tools for the multiplexed analysis of small molecule- and peptide-protein interactions. However, these arrays are technically difficult to manufacture and use with high reproducibility and require specialized equipment. Here we report a more convenient alternative composed of color-encoded beads that display a small molecule protein ligand on the surface. Quantitative, multiplexed assay of protein binding to up to 24 different ligands can be achieved using a common flow cytometer for the readout. This technology should be useful for evaluating hits from library screening efforts, the determination of structure activity relationships, and certain types of serological analyses.

PMID:
24245981
[PubMed - indexed for MEDLINE]
PMCID:
PMC3944025
Free PMC Article
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