Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2013 Nov 7;8(11):e78420. doi: 10.1371/journal.pone.0078420. eCollection 2013.

Interaction with intestinal epithelial cells promotes an immunosuppressive phenotype in Lactobacillus casei.

Author information

  • 1Advanced Therapies and Product Development, Finnish Red Cross Blood Service, Helsinki, Finland.

Abstract

Maintenance of the immunological tolerance and homeostasis in the gut is associated with the composition of the intestinal microbiota. We here report that cultivation of Lactobacillus casei ATCC 334 in the presence of human intestinal epithelial cells promotes functional changes in bacteria. In particular, the interaction enhanced the immunosuppressive phenotype of L. casei as demonstrated by the ability of L. casei to generate functional regulatory T cells (CD4+CD25+FoxP3+) and production of the anti-inflammatory cytokine interleukin-10 by human peripheral blood mononuclear cells. The results indicate microbe-host cross-talk that changes features of microbes, and suggest that in vitro simulation of epithelial cell interaction can reveal functional properties of gut microbes more accurately than conventional cultivation.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk