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Oncogene. 2014 Nov 20;33(47):5434-41. doi: 10.1038/onc.2013.490. Epub 2013 Nov 18.

Novel fusion transcripts in human gastric cancer revealed by transcriptome analysis.

Author information

  • 1Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
  • 21] Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea [2] Department of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea.
  • 31] Genomic Medicine Institute, Medical Research Center, Seoul National University, Seoul, Korea [2] Psoma Therapeutic Inc, Seoul, Korea.
  • 41] Genomic Medicine Institute, Medical Research Center, Seoul National University, Seoul, Korea [2] Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Korea.
  • 5Department of Chemistry, College of Science, Yonsei University, Seoul, Korea.
  • 61] Genomic Medicine Institute, Medical Research Center, Seoul National University, Seoul, Korea [2] Psoma Therapeutic Inc, Seoul, Korea [3] Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Korea [4] Department of Biochemistry, Seoul National University College of Medicine, Seoul, Korea [5] Macrogen Inc., Seoul, Korea.
  • 71] Genomic Medicine Institute, Medical Research Center, Seoul National University, Seoul, Korea [2] Psoma Therapeutic Inc, Seoul, Korea [3] Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Korea [4] Department of Biochemistry, Seoul National University College of Medicine, Seoul, Korea.
  • 81] Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea [2] Department of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea [3] Department of Molecular Medicine & Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University College of Medicine, Seoul, Korea.

Abstract

Gene fusion is involved in the development of various types of malignancies. Recent advances in sequencing technology have facilitated identification of gene fusions and have stimulated the research of this field in cancer. In the present study, we performed next-generation transcriptome sequencing in order to discover novel gene fusions in gastric cancer. A total of 282 fusion transcript candidates were detected from 12 gastric cancer cell lines by bioinformatic filtering. Among the candidates, we have validated 19 fusion transcripts, which are 7 inter-chromosomal and 12 intra-chromosomal fusions. A novel DUS4L-BCAP29 fusion transcript was found in 2 out of 12 cell lines and 10 out of 13 gastric cancer tissues. Knockdown of DUS4L-BCAP29 transcript using siRNA inhibited cell proliferation. Soft agar assay further confirmed that this novel fusion transcript has tumorigenic potential. We also identified that microRNA-coding gene PVT1, which is amplified in double minute chromosomes in SNU-16 cells, is recurrently involved in gene fusion. PVT1 produced six different fusion transcripts involving four different genes as fusion partners. Our findings provide better insight into transcriptional and genetic alterations of gastric cancer: namely, the tumorigenic effects of transcriptional read-through and a candidate region for genetic instability.

[PubMed - indexed for MEDLINE]
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