Titanium-adhesive polymer nanoparticles as a surface-releasing system of dual osteogenic growth factors

Macromol Biosci. 2014 Apr;14(4):496-507. doi: 10.1002/mabi.201300368. Epub 2013 Nov 12.

Abstract

A titanium (Ti)-adhesive nanoparticle is developed as a surface-releasing system for dual osteogenic growth factors. The Ti-adhesive nanoparticle is prepared by self-assembly of a poly(L-lactide-co-glycolide) (PLGA)-grafted hyaluronic acid (HA) copolymer, followed by conjugation of catechol groups on nanoparticle surfaces. The nanoparticles consist of Ti-adhesive peripheral catechol groups, anionic HA shells, and hydrophobic PLGA inner cores. The immobilization of the nanoparticles onto Ti substrates is successfully verified using various analytical tools including field-emission scanning electron microscopy (Fe-SEM), contact angle measurement, and X-ray photoelectron spectroscopy (XPS). Positively charged dual growth factors, bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) are readily loaded onto the negatively charged HA shells of surface-immobilized nanoparticles, which is confirmed by fluorescence microscopy. The Ti substrates with dual growth factor-loaded nanoparticle-immobilized nanoparticles remarkably promote the attachment, proliferation, spreading, and alkaline phosphatase (ALP) activity of human adipose-derived stem cells (hADSCs).

Keywords: adhesive nanoparticles; bone morphogenetic protein-2; controlled release; insulin-like growth factor-1; surface treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesives / chemistry*
  • Bone Morphogenetic Protein 2 / metabolism
  • Cell Proliferation / genetics*
  • Humans
  • Hyaluronic Acid / chemistry
  • Hyaluronic Acid / therapeutic use
  • Insulin-Like Growth Factor I / metabolism
  • Lactic Acid / chemistry
  • Lactic Acid / therapeutic use
  • Microscopy, Electron, Scanning
  • Nanoparticles / chemistry*
  • Osteoblasts / ultrastructure
  • Polyglycolic Acid / chemistry
  • Polyglycolic Acid / therapeutic use
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Surface Properties
  • Titanium / chemistry

Substances

  • Adhesives
  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Insulin-Like Growth Factor I
  • Hyaluronic Acid
  • Titanium