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Biochem Biophys Res Commun. 2013 Nov 29;441(4):935-40. doi: 10.1016/j.bbrc.2013.10.166. Epub 2013 Nov 9.

Alpha-lipoic acid protects cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy.

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  • 1Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Abstract

Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Alpha-lipoic acid (LA) plays an important role in the etiology of cardiovascular disease. Autophagy is widely implicated in myocardial I/R injury. We assessed the degree of autophagy by pretreatment with LA exposed to H/R in H9c2 cell based on the expression levels of Beclin-1, LC3II/LC3I, and green fluorescent protein-labeled LC3 fusion proteins. Autophagic vacuoles were confirmed in H9c2 cells exposed to H/R using transmission electron microscopy. Our findings indicated that pretreatment with LA inhibited the degree of autophagy in parallel to the enhanced cell survival and decreased total cell death in H9c2 cells exposed to H/R. We conclude that LA protects cardiomyocytes against H/R injury by inhibiting autophagy.

Copyright © 2013. Published by Elsevier Inc.

KEYWORDS:

Alpha-lipoic acid; Autophagy; Cardiomyocytes; Hypoxia/reoxygenation

PMID:
24216106
[PubMed - indexed for MEDLINE]
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