Comparative volatile profiles in soy sauce according to inoculated microorganisms

Biosci Biotechnol Biochem. 2013;77(11):2192-200. doi: 10.1271/bbb.130362. Epub 2013 Nov 7.

Abstract

We compared the volatile profiles in soy sauce according to inoculation with Tetragenococcus halophilus and/or Zygosaccharomyces rouxii. Totals of 107 and 81 volatiles were respectively identified by using solid-phase microextraction and solvent extraction. The various volatile compounds identified included acids, aldehydes, esters, ketones, furans and furan derivatives, and phenols. The major volatiles in the samples treated with T. halophilus were acetic acid, formic acid, benzaldehyde, methyl acetate, ethyl 2-hydroxypropanoate, 2-hydroxy-3-methyl-2-cyclopenten-1-one, and 4-hydroxy-3-methoxybenzaldehyde, while those in the samples inoculated with Z. rouxii were mainly ethanol, acetaldehyde, ethyl propanoate, 2/3-methylbutanol, 1-butanol, 2-phenylethanol, ethyl 2-methylpropanoate, and 4-hydroxy-2-ethyl-5-methyl-3(2H)-furanone. The results indicate that T. halophilus produced significant acid compounds and could affect the Z. rouxii activity, supporting the notion that yeasts and lactic acid bacteria respectively have different metabolic pathways of alcoholic fermentation and lactic acid fermentation, and produce different dominant volatile compounds in soy sauce.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohols / analysis
  • Alcohols / metabolism
  • Aldehydes / analysis
  • Aldehydes / metabolism
  • Carboxylic Acids / analysis
  • Carboxylic Acids / metabolism
  • Enterococcaceae / metabolism*
  • Fermentation
  • Furans / analysis
  • Furans / metabolism
  • Metabolic Networks and Pathways
  • Phenols / analysis
  • Phenols / metabolism
  • Solid Phase Microextraction
  • Solvents
  • Soy Foods / analysis
  • Soy Foods / microbiology*
  • Species Specificity
  • Terpenes / analysis
  • Terpenes / metabolism
  • Volatile Organic Compounds / analysis*
  • Volatile Organic Compounds / metabolism
  • Zygosaccharomyces / metabolism*

Substances

  • Alcohols
  • Aldehydes
  • Carboxylic Acids
  • Furans
  • Phenols
  • Solvents
  • Terpenes
  • Volatile Organic Compounds