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Theriogenology. 2014 Jan 15;81(2):243-9. doi: 10.1016/j.theriogenology.2013.09.018. Epub 2013 Oct 21.

Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos.

Author information

  • 1Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
  • 2College of Natural and Mathematical Sciences, University of Maryland, Baltimore County, Baltimore, Maryland, USA.
  • 3College of Arts and Science, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
  • 4College of Carnegie Institute of Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
  • 5Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, Canada.
  • 6College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
  • 7College of Engineering, University of Michigan, Ann Arbor, Michigan, USA.
  • 8Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea. Electronic address: bclee@snu.ac.kr.

Abstract

Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures possess different capacities to support pre-implantation development of SCNT embryos. The aim of this study is to investigate whether two culture medium (RCMEP, Dulbecco's modified Eagle's medium [DMEM]) affect gene expression of ASCs, subsequent development of interspecies SCNT (iSCNT) and gene expression of cloned embryos. The RCMEP-cultured cells contained significantly greater amounts of SOX2, NANOG, OCT4, DNMT1, and MeCP2 than DMEM-cultured cells (P < 0.05). In iSCNT, the use of DMEM medium for culturing cells resulted in similar development to the blastocyst stage than those derived from RCMEP cultured cells (4.5% and 3.2%, respectively; P > 0.05). The expression of all transcripts except for DNMT1 in cloned blastocysts from RCMEP cultured cells followed those of cloned blastocysts derived from DMEM cultured cells. The alteration of gene expression in ASCs by culture medium was not manifested in the iSCNT embryos derived from these cells. Although the culture medium can induce changes of gene expression by ASCs, such alterations in donor cells did not affect the developmental competence or gene expression patterns of iSCNT embryos.

Copyright © 2014. Published by Elsevier Inc.

KEYWORDS:

Canine adipose-derived mesenchymal stem cells; DMEM; Gene expression; RCMEP; iSCNT; iSCNT blastocyst

PMID:
24157230
[PubMed - indexed for MEDLINE]
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