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Bull Exp Biol Med. 2013 Aug;155(4):413-6.

Contrasting of biological samples for X-ray synchrotron microtomography.

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  • 1Laboratory of Neurobiology of Memory, P. K. Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences; Neurocognitive NTK, Kurchatov NBIC-center, National Research Centre "Kurchatov Institute"; Synchrotron and Neutron NTK, Kurchatov NBIC-center, National Research Centre "Kurchatov Institute", Moscow, Russia. Efimova_OI@nrcki.ru.

Abstract

The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed by a 48-h washout in phosphate buffered saline had maximum contrast and lowest compression artifacts. Processing of samples by this protocol allowed detecting zones of active proliferation. Incubation of brain samples for 120 h in 7.6% meglumine/sodium diatrizoate without washout ensured the best contrast during myelin identification.

PMID:
24143358
[PubMed - indexed for MEDLINE]
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