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J Immunol Methods. 2013 Dec 31;400-401:117-21. doi: 10.1016/j.jim.2013.10.005. Epub 2013 Oct 17.

Quantification of secretory leukocyte protease inhibitor (SLPI) in oral gargle specimens collected using mouthwash.

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  • 1Department of Cancer Epidemiology, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA; Center for Infection Research in Cancer, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA.

Abstract

BACKGROUND:

Secretory leukocyte protease inhibitor (SLPI) is an innate immunity-associated protein known to inhibit HIV transmission, and is thought to inhibit a variety of infectious agents, including human papillomaviruses (HPVs). We aimed to optimize an established ELISA-based SLPI quantification assay for use with oral gargle specimens collected using mouthwash, and to assess preliminary associations with age, smoking status, and alcohol intake.

METHODS:

Oral gargle supernatants from 50 individuals were used to optimize the Human SLPI Quantikine ELISA Kit. Sample suitability was assessed and quality control analyses were conducted.

RESULTS:

Salivary SLPI was successfully recovered from oral gargles with low intra-assay and high inter-individual variability. Initial measurements showed that salivary SLPI varied considerably across individuals, and that SLPI was inversely associated with age.

CONCLUSIONS:

This optimized assay can be used to examine the role of SLPI in the acquisition of oral HPV and other infections.

© 2013. Published by Elsevier B.V. All rights reserved.

KEYWORDS:

CV; HNC; HPV; Immune system protein; Innate immunity; OD; Oral disease; Oral gargle; SLPI; Secretory leukocyte protease inhibitor; coefficients of variation.; head and neck cancer; human papillomavirus; optical density; secretory leukocyte protease inhibitor

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