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J Immunol Methods. 2013 Dec 31;400-401:117-21. doi: 10.1016/j.jim.2013.10.005. Epub 2013 Oct 17.

Quantification of secretory leukocyte protease inhibitor (SLPI) in oral gargle specimens collected using mouthwash.

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  • 1Department of Cancer Epidemiology, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA; Center for Infection Research in Cancer, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA.



Secretory leukocyte protease inhibitor (SLPI) is an innate immunity-associated protein known to inhibit HIV transmission, and is thought to inhibit a variety of infectious agents, including human papillomaviruses (HPVs). We aimed to optimize an established ELISA-based SLPI quantification assay for use with oral gargle specimens collected using mouthwash, and to assess preliminary associations with age, smoking status, and alcohol intake.


Oral gargle supernatants from 50 individuals were used to optimize the Human SLPI Quantikine ELISA Kit. Sample suitability was assessed and quality control analyses were conducted.


Salivary SLPI was successfully recovered from oral gargles with low intra-assay and high inter-individual variability. Initial measurements showed that salivary SLPI varied considerably across individuals, and that SLPI was inversely associated with age.


This optimized assay can be used to examine the role of SLPI in the acquisition of oral HPV and other infections.

© 2013. Published by Elsevier B.V. All rights reserved.


CV; HNC; HPV; Immune system protein; Innate immunity; OD; Oral disease; Oral gargle; SLPI; Secretory leukocyte protease inhibitor; coefficients of variation.; head and neck cancer; human papillomavirus; optical density; secretory leukocyte protease inhibitor

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