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Eur J Nutr. 2014 Apr;53(3):897-906. doi: 10.1007/s00394-013-0593-8. Epub 2013 Oct 16.

Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect.

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  • 1Departamento de Patología, Facultad de Medicina, Centro de Patología Experimental y Aplicada (CPEA), Universidad de Buenos Aires, J. E. Uriburu 950, 5º piso, (C1114AAD), Ciudad Autónoma de Buenos Aires, Argentina, vdenninghoff@cemic.edu.ar.

Abstract

PURPOSE:

The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model.

METHODS:

Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed.

RESULTS:

All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats.

CONCLUSIONS:

This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury.

PMID:
24129499
[PubMed - indexed for MEDLINE]
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