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Angew Chem Int Ed Engl. 2013 Dec 2;52(49):13020-3. doi: 10.1002/anie.201305461. Epub 2013 Oct 11.

Multiplex detection of DNA mutations by the fluorescence fingerprint spectrum technique.

Author information

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China).

Abstract

A fingerprint spectrum technique that utilizes cationic conjugated-polymer-based fluorescence resonance energy transfer (FRET) is used for multiplex detection of DNA mutations. This method detects as low as 5 % mutation of the total DNA. Ten PIK3CA mutations originating from 30 clinical breast cancer samples could be detected.

Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KEYWORDS:

DNA mutation; FRET; biosensors; conjugated polymers; fingerprint spectrum

PMID:
24127406
[PubMed - in process]
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