A molecular clone of HTLV-III with biological activity

Nature. 1985 Jul;316(6025):262-5. doi: 10.1038/316262a0.

Abstract

Acquired immune deficiency syndrome (AIDS) is an epidemic immunosuppressive disease characteristically associated with a depletion of T lymphocytes of the helper/inducer phenotype. Numerous converging lines of research have implicated a human T-cell lymphotropic retrovirus, HTLV-III, in the pathogenesis of AIDS. Recently, several distinct forms of the HTLV-III genome were molecularly cloned in phage and extensively characterized. In the present study, a clone containing full-length HTLV-III proviral DNA was inserted into a plasmid and used to transfect cord blood T cells from normal newborn humans. We demonstrate that this molecular clone is infectious in vitro and causes marked cytopathic effects on T-cell cultures. This is the first direct evidence that the HTLV-III genome, rather than a minor component of the virus complex, is cytopathic for T cells. Using this biologically competent clone and mutants derived from it, it should now be possible to localize the subgenomic regions that contribute to the biological effects of HTLV-III.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / microbiology
  • Antigens, Viral / genetics
  • Cells, Cultured
  • Cloning, Molecular
  • Cytopathogenic Effect, Viral
  • DNA, Viral / biosynthesis
  • Deltaretrovirus / genetics*
  • Gene Products, gag
  • Humans
  • RNA-Directed DNA Polymerase / metabolism
  • T-Lymphocytes / microbiology*
  • Transfection
  • Viral Proteins / genetics
  • Virus Replication

Substances

  • Antigens, Viral
  • DNA, Viral
  • Gene Products, gag
  • Viral Proteins
  • RNA-Directed DNA Polymerase