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J Neurosci Res. 2013 Dec;91(12):1572-80. doi: 10.1002/jnr.23285. Epub 2013 Sep 16.

The mannose receptor is expressed by olfactory ensheathing cells in the rat olfactory bulb.

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  • 1Instituto de Biof√≠sica Carlos Chagas Filho, Laboratory of Comparative and Developmental Neurobiology, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.

Abstract

Complex carbohydrate structures are essential molecules of infectious bacteria, parasites, and host cells and are involved in cell signaling associated with immune responses, glycoprotein homeostasis, and cell migration. The uptake of mannose-tailed glycans is usually carried out by professional phagocytes to trigger MHC class I- and MHC class II-restricted antigen presentation or, alternatively, to end inflammation. We have detected the mannose receptor (MR) in cultured olfactory ensheathing cells (OECs), so we investigated by flow cytometry whether recently dissociated cells of the olfactory bulb (OB) nerve fiber layer (ONL) could bind a mannosylated ligand (fluorescein conjugate of mannosyl bovine serum albumin; Man/BSA-FITC) in a specific manner. In addition, we estimated the relative proportion of ONL OECs, microglia, and astrocytes, tagged by 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), by the B4 isolectin of Griffonia simplicifonia (IB4), and by glial fibrillary acidic protein (GFAP), respectively, that were Man/BSA-FITC(+) . We also determined by histochemistry and/or immunohistochemistry whether Man/BSA-FITC or an anti-MR antibody (anti-C-terminal MR peptide; anti-cMR) labeled OECs and/or parenchymal microglia. In addition, we confirmed by Western blot with the K1K2 (against the entire MR molecule) antibody that a band of about 180 kDA is expressed in the OB. Our findings are compatible with a prospective sentinel role of OECs against pathogens of the upper airways and/or damage-associated glycidic patterns as well as with homeostasis of OB mannosylated glycoproteins.

Copyright © 2013 Wiley Periodicals, Inc.

KEYWORDS:

C-type lectin receptors; endocytosis; inflamation; microglia; neuroglial cells; phagocytosis

PMID:
24105692
[PubMed - indexed for MEDLINE]
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