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Vet J. 2013 Nov;198(2):504-7. doi: 10.1016/j.tvjl.2013.08.032. Epub 2013 Sep 6.

Evaluation of an in-clinic assay for the diagnosis of canine parvovirus.

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  • 1Department of Veterinary Medicine, University of Bari, Valenzano (Bari), Italy. Electronic address: nicola.decaro@uniba.it.

Abstract

The results of a study designed to evaluate the performance of an in-clinic test for the detection of canine parvovirus (CPV) are reported. A total of 150 faecal samples collected from dogs with acute diarrhoea were tested using the in-clinic test, a haemagglutination assay (HA) and a real-time PCR assay for CPV detection, quantification and characterisation. CPV was detected in 66, 73, and 101 faecal samples by in-clinic, HA and PCR testing, respectively. The relative sensitivity and specificity of the in-clinic test were 86.3% and 96.1%, respectively, when the test was compared to HA, and 65.3% and 100%, respectively, when compared to real-time PCR. The sample distribution according to the virus type was CPV-2a, n=44; CPV-2b, n=11; CPV-2c, n=44, CPV-2, n=2, as determined by minor groove binder probe assays and/or sequence analysis. The percentage of positive in-clinic tests was 70.5% for CPV-2a, 72.7% for CPV-2b and 75.0% for CPV-2c (P>0.05). Using real-time PCR as the reference standard for CPV detection, the in-clinic test was more specific than HA and had comparable sensitivity to HA, demonstrating detection rates similar to those previously observed for other rapid in-clinic tests. The in-clinic test was also able to detect all CPV types at equivalent rates.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

CPV-2c; Canine parvovirus; Diagnosis; In-clinic test

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