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Genome Biol. 2013;14(10):R109.

High-efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing.

Abstract

Small RNA cloning and sequencing is uniquely positioned as a genome-wide approach to quantify miRNAs with single-nucleotide resolution. However, significant biases introduced by RNA ligation in current protocols lead to inaccurate miRNA quantification by 1000-fold. Here we report an RNA cloning method that achieves over 95% efficiency for both 5′ and 3′ ligations. It achieves accurate quantification of synthetic miRNAs with less than two-fold deviation from the anticipated value and over a dynamic range of four orders of magnitude. Taken together, this high-efficiency RNA cloning method permits accurate genome-wide miRNA profiling from total RNAs.

PMID:
24098942
[PubMed - indexed for MEDLINE]
PMCID:
PMC3983620
Free PMC Article

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