FEBS Lett. 1985 Jul 8;186(2):168-74.

1H-NMR study of gramicidin A transmembrane ion channel. Head-to-head right-handed, single-stranded helices.

Arseniev AS, Barsukov IL, Bystrov VF, Lomize AL, Ovchinnikov YuA.

Abstract

The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in detergent micelles, which in many ways mimic the phospholipid bilayer of biomembranes, is an N-terminal to N-terminal (head-to-head) dimer (Formula: see text) formed by two right-handed, single-stranded beta 6.3 helices with 6.3 residues per turn, differing from Urry's structure by handedness of the helices.

PMID:: 2408920; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Refinement Of The Spatial Structure Of The Gramicidin A Transmembrane Ion-Channel (Russian)

PDB: 1GRM

Source: Brevibacillus brevis

Method: Solution Nmr

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