Send to:

Choose Destination
See comment in PubMed Commons below
J Nat Sci Biol Med. 2013 Jul;4(2):435-9. doi: 10.4103/0976-9668.117018.

Liquid chromatographic determinations of meta-chlorobenzoic acid in bupropion hydrochloride.

Author information

  • 1Department of Analytical Research Lab, Glenmark Generics Ltd, Glenmark Research Centre, Plot A - 607, TTC Industrial Area, MIDC, Mahape, Navi Mumbai, Maharashtra, India.


A simple, sensitive method for the determination of meta-chlorobenzoic acid in bupropion hydrochloride is described. Chromatographic separation of m-chlorobenzoic acid is achieved using a mobile phase consisting of n-hexane and ethanol (1000:50, v/v) at a flow rate of 1.0 ml/min on a Chiralpak ADH (250 × 4.6 mm). Absorbance is monitored at 235 nm. The method is linear for m-chlorobenzoic acid over concentration range of LOQ, 5.0 μg/ml to 15.0 μg/ml for m-chlorobenzoic acid with correlation coefficient greater than 0.99. This method is more selective and accurate than United States Pharmacopoeia method for the determination of m-chlorobenzoic acid in bupropion hydrochloride.


1-(3-Chlorophenyl)-1,2-propanedione; HPLC method; bupropion Hcl; meta-chlorobenzoic acid; method validation

Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk