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J Nat Sci Biol Med. 2013 Jul;4(2):435-9. doi: 10.4103/0976-9668.117018.

Liquid chromatographic determinations of meta-chlorobenzoic acid in bupropion hydrochloride.

Author information

  • 1Department of Analytical Research Lab, Glenmark Generics Ltd, Glenmark Research Centre, Plot A - 607, TTC Industrial Area, MIDC, Mahape, Navi Mumbai, Maharashtra, India.

Abstract

A simple, sensitive method for the determination of meta-chlorobenzoic acid in bupropion hydrochloride is described. Chromatographic separation of m-chlorobenzoic acid is achieved using a mobile phase consisting of n-hexane and ethanol (1000:50, v/v) at a flow rate of 1.0 ml/min on a Chiralpak ADH (250 × 4.6 mm). Absorbance is monitored at 235 nm. The method is linear for m-chlorobenzoic acid over concentration range of LOQ, 5.0 μg/ml to 15.0 μg/ml for m-chlorobenzoic acid with correlation coefficient greater than 0.99. This method is more selective and accurate than United States Pharmacopoeia method for the determination of m-chlorobenzoic acid in bupropion hydrochloride.

KEYWORDS:

1-(3-Chlorophenyl)-1,2-propanedione; HPLC method; bupropion Hcl; meta-chlorobenzoic acid; method validation

PMID:
24082747
[PubMed]
PMCID:
PMC3783795
Free PMC Article
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