Characterization of oral Helicobacter pylori strain by 4 methods

Lyudmila Boyanova; Vladimir Panov; Daniel Yordanov; Galina Gergova; Ivan Mitov

doi:10.1016/j.diagmicrobio.2013.06.030

Characterization of oral Helicobacter pylori strain by 4 methods

Diagn Microbiol Infect Dis. 2013 Dec;77(4):287-8. doi: 10.1016/j.diagmicrobio.2013.06.030. Epub 2013 Sep 26.

Authors

Lyudmila Boyanova¹, Vladimir Panov, Daniel Yordanov, Galina Gergova, Ivan Mitov

Affiliation

¹ Department of Medical Microbiology, Medical University of Sofia, 1431 Sofia, Bulgaria. Electronic address: l.boyanova@hotmail.com.

PMID: 24075629
DOI: 10.1016/j.diagmicrobio.2013.06.030

Abstract

The aim of the study was to describe oral Helicobacter pylori strain from a child by 4 methods. The strain was positive by immunofluorescence, ureA- and cagA positive, vacA s1 m2 genotype and resistant to metronidazole and clarithromycin. In conclusion, virulent and antibiotic resistant H. pylori strains can be present in oral cavity from patients with chronic dental and gastroduodenal diseases.

Keywords: Children; Helicobacter pylori; Immunofluorescence; Isolation; Oral cavity; PCR; Resistance; Virulence.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Proteins / immunology
Bacterial Typing Techniques*
Clarithromycin / pharmacology
Genotype
Helicobacter Infections / microbiology*
Helicobacter pylori / classification*
Helicobacter pylori / drug effects
Helicobacter pylori / genetics
Humans
Metronidazole / pharmacology
Microbial Sensitivity Tests
Mouth / microbiology*
Polymerase Chain Reaction
Virulence Factors / genetics
Virulence Factors / immunology

Substances

Anti-Bacterial Agents
Bacterial Proteins
Virulence Factors
Metronidazole
Clarithromycin