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Nan Fang Yi Ke Da Xue Xue Bao. 2013 Sep;33(9):1316-20.

[Effect of propofol on thrombospondin-1 expression in cultured newborn rat cortical astrocytes].

[Article in Chinese]

Author information

  • 1Department of Anesthesiology, Peiking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.E-mial: squirrelyuki@126.com.

Abstract

OBJECTIVE:

To investigate the effect of propofol on the expression of thrombospondin-1 (THBS-1) mRNA and protein in purified newborn rat cortical astrocytes in vitro.

METHODS:

Astrocytes were isolated from newborn rat cortex and grown in culture before exposure to propofol at 3, 10, 30, 100 or 300 ┬Ámol/L for 6 h, 12, or 24 h. The mRNA level of THBS-1 was detected by RT-PCR, and the protein level of THBS-1 was detected by immunofluorescence cytochemistry and Western blotting.

RESULTS:

Propofol exposure caused significantly upregulated THBS-1 level in cultured astrocytes (P<0.05) to a level about 1.3 times higher than that in control cells. The mRNA and protein levels of THBS-1 in cultured rat cortical astrocytes were upregulated by exposures to 10, 30 and 100 ┬Ámol/L propofol (P<0.01). High expression of THBS-1 mRNA and protein was detected in the cells with exposures for different durations (P<0.05), especially in the 12 h group (P<0.01).

CONCLUSION:

Propofol at clinically relevant concentrations can modulate the level of THBS-1 secreted by astrocytes of rat cerebral cortex in vitro.

PMID:
24067211
[PubMed - indexed for MEDLINE]
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