Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Dev Biol. 2013 Nov 15;383(2):264-74. doi: 10.1016/j.ydbio.2013.09.010. Epub 2013 Sep 19.

Eph:ephrin-B1 forward signaling controls fasciculation of sensory and motor axons.

Author information

  • 1Centre de Biologie du Développement, CNRS, 118 Route de Narbonne, 31062 Toulouse, France; UPS, Université de Toulouse, France.

Abstract

Axon fasciculation is one of the processes controlling topographic innervation during embryonic development. While axon guidance steers extending axons in the accurate direction, axon fasciculation allows sets of co-extending axons to grow in tight bundles. The Eph:ephrin family has been involved both in axon guidance and fasciculation, yet it remains unclear how these two distinct types of responses are elicited. Herein we have characterized the role of ephrin-B1, a member of the ephrinB family in sensory and motor innervation of the limb. We show that ephrin-B1 is expressed in sensory axons and in the limb bud mesenchyme while EphB2 is expressed in motor and sensory axons. Loss of ephrin-B1 had no impact on the accurate dorso-ventral innervation of the limb by motor axons, yet EfnB1 mutants exhibited decreased fasciculation of peripheral motor and sensory nerves. Using tissue-specific excision of EfnB1 and in vitro experiments, we demonstrate that ephrin-B1 controls fasciculation of axons via a surround repulsion mechanism involving growth cone collapse of EphB2-expressing axons. Altogether, our results highlight the complex role of Eph:ephrin signaling in the development of the sensory-motor circuit innervating the limb.

© 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Axon fasciculation; Axon guidance; Ephrin; Motor neurons; Mouse; Sensory neurons

PMID:
24056079
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk