Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2014;1071:163-74. doi: 10.1007/978-1-62703-622-1_13.

Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity.

Author information

  • 1Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, IL, USA.


The proteolytic activity of Membrane-type 1 Matrix Metalloproteinase (MT1-MMP) is crucial for cancer cell invasion and metastasis. To visualize the protease activity of MT1-MMP with high spatiotemporal resolution at the extracellular plasma membrane surface of live cancer cells, a genetically encoded fluorescent biosensor of MT1-MMP has been developed. Here we describe the design principles of the MT1-MMP biosensor, the characterization of the MT1-MMP biosensor in vitro, and the live-cell imaging protocol used to visualize MT1-MMP activity in mammalian cells. We also provide brief guidelines for observing MT1-MMP subcellular activity by fluorescence resonance energy transfer (FRET) in a cell migration assay.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk