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J Microbiol Methods. 2013 Nov;95(2):251-2. doi: 10.1016/j.mimet.2013.09.001. Epub 2013 Sep 17.

Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus.

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  • 1Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Applied Wood Materials, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland. Electronic address: mark.schubert@empa.ch.


The biotechnologically important white-rot fungus Physisporinus vitreus was co-cultivated with Agrobacterium tumefaciens AGL-1 carrying plasmids with nourseothricin resistance as the selectable marker gene and red fluorescence protein as a visual marker. Mitotically stable transformed isolates were obtained showing red fluorescence protein activity.

© 2013 Elsevier B.V. All rights reserved.


Agrobacterium tumefaciens; Physisporinus vitreus; Red fluorescent protein (RFP); Transformation

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