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Chem Commun (Camb). 2013 Oct 3;49(86):10085-7. doi: 10.1039/c3cc44017f.

5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase.

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  • 1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn srwang@whu.edu.cn.

Abstract

Here, we first demonstrated that 5-MedCTP could be incorporated into the synthetic DNA template by the exonuclease deficient Klenow fragment with a much higher efficiency than dCTP and 5-hydroxymethyl-dCTP. Further, we first conducted a comparable study of primer extension reaction using templates containing deoxycytidine (dC) or 5-methyldeoxycytidine (5-mdC) for incorporating different triphosphates. Based on our findings, 5-methyldeoxycytidine could enhance the substrate activity of the Klenow fragment (exo-) and this feature could potentially be used in DNA methylation analysis.

PMID:
24045613
[PubMed - indexed for MEDLINE]
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