De novo assembly of the peanut (Arachis hypogaea L.) seed transcriptome revealed candidate unigenes for oil accumulation pathways

Dongmei Yin; Yun Wang; Xingguo Zhang; Hemin Li; Xiang Lu; Jinsong Zhang; Wanke Zhang; Shouyi Chen

doi:10.1371/journal.pone.0073767

De novo assembly of the peanut (Arachis hypogaea L.) seed transcriptome revealed candidate unigenes for oil accumulation pathways

PLoS One. 2013 Sep 10;8(9):e73767. doi: 10.1371/journal.pone.0073767. eCollection 2013.

Authors

Dongmei Yin¹, Yun Wang, Xingguo Zhang, Hemin Li, Xiang Lu, Jinsong Zhang, Wanke Zhang, Shouyi Chen

Affiliation

¹ Henan Agricultural University, Zhengzhou, China ; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Abstract

Peanuts are one of the most important edible oil crops in the world. In order to survey key genes controlling peanut oil accumulation, we analyzed the seed transcriptome in different developmental stages of high- and low-oil peanut varieties. About 54 million high quality clean reads were generated, which corresponded to 4.85 Gb total nucleotides. These reads were assembled into 59,236 unique sequences. Differential mRNA processing events were detected for most of the peanut Unigenes and found that 15.8% and 18.0% of the Unigenes were differentially expressed between high- and low-oil varieties at 30 DAF and 50 DAF, respectively. Over 1,500 Unigenes involved in lipid metabolism were identified, classified, and found to participate in FA synthesis and TAG assembly. There were seven possible metabolic pathways involved in the accumulation of oil during seed development. This dataset provides more sequence resource for peanut plant and will serve as the foundation to understand the mechanisms of oil accumulation in oil crops.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arachis / genetics*
Arachis / growth & development
Arachis / metabolism
Cluster Analysis
Gene Expression Profiling / methods
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Ontology
Genes, Plant / genetics*
Lipid Metabolism / genetics
Metabolic Networks and Pathways / genetics*
Peanut Oil
Plant Oils / metabolism*
Plant Proteins / genetics
Plant Proteins / metabolism
Seeds / genetics*
Seeds / growth & development
Seeds / metabolism
Time Factors
Transcriptome*

Substances

Peanut Oil
Plant Oils
Plant Proteins

Grants and funding

This work was supported by the National Key Basic Research Projects (2011CB109301 and 2010CB125903), National Transgenic Research Projects (2013ZX08004-003), Program for New Century Excellent Talents in University and the National Natural Science Funds (NCET-09-0121) and the National Natural Science Foundation of China (U1204317). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.