[Detection of four parasitic infections using ELISA]

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Jun;25(3):327-8.
[Article in Chinese]

Abstract

Objective: To study the detection effects of ELISA for the infections of Cysticercus, Echinococcosus, Paragonimus westermani and Sparganum mansoni in order to improve diagnosis.

Methods: The patients who admitted to Jiangsu Institute of Parasitic Diseases from 2008 to 2001 were selected as the subjects and their sera were detected for IgG against Cysticercus, Echinococcosus, and Paragonimus westermani from January 2008 to March 2011, and IgG against Sparganum mansoni since June, 2011 by ELISA.

Results: A total of 282 cases were detected, and 88 of them were found one or two positive results, and the total positive rate was 31.2%. There were 39, 2, 21, and 3 cases of positive IgG of Cysticercus, Echinococcosus, Paragonimus westermani and Sparganum mansoni, respectively. There were 15 cases of positive IgG to both Cysticercus and Echinococcosus; there were 5 cases of positive IgG to both Cysticercus and Paragonimus westermani; there were 3 cases of positive IgG to both Echinococcosus and Paragonimus westermani. The cross reactions occurred in 23 serum samples among 88 positive samples with a cross reaction rate of 26.1%.

Conclusion: There still exist a certain number of patients with parasitic diseases, and the cross reaction may occur in the detection process of IgG to Cysticercus, Echinococcosus, Paragonimus westermani and Sparganum mansoni by ELISA.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antibodies, Helminth / blood*
  • Child
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Helminthiasis / blood*
  • Helminthiasis / diagnosis
  • Helminthiasis / parasitology
  • Helminths / immunology
  • Helminths / isolation & purification*
  • Humans
  • Immunoglobulin G / blood
  • Male
  • Middle Aged
  • Young Adult

Substances

  • Antibodies, Helminth
  • Immunoglobulin G