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Wei Sheng Yan Jiu. 2013 Jul;42(4):550-3, 570.

[Inhibitory effects of exogenous zinc on enzymatic activity of tumor suppressor protein PTEN and possible mechanisms in human airway epithelial cells].

[Article in Chinese]

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  • 1College of Public Health, Zhengzhou University, Zhengzhou 450001, China.



To examine the effect of exogenous zinc on enzymatic activity of tumor suppressor PTEN and underlying mechanisms.


Human airway epithelial cells (BEAS-2B) were employed as the in vitro model. Enzymatic activity of PTEN was measured using malachite green colorimetric assay and immunoblotting. Moreover, the activity of casein kinase-2 (CK-2), the upstream kinase of PTEN, was determined.


Exposure of BEAS-2B cells to Zn2+ inhibited phosphatase activity of PTEN, as well as PTEN phosphorylation, indicative of PTEN activity (P < 0.05). Under the same experimental condition, Zn2+ stimulation resulted in significant reduction of CK-2 activity (P < 0.05). In addition, CK-2 was shown to induce PTEN phosphorylation (P < 0.05).


These results suggested that Zn2+ exposure might inhibit PTEN phosphatase activity through inhibition of CK-2 activity in human airway epithelial cells.

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