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Wei Sheng Yan Jiu. 2013 Jul;42(4):550-3, 570.

[Inhibitory effects of exogenous zinc on enzymatic activity of tumor suppressor protein PTEN and possible mechanisms in human airway epithelial cells].

[Article in Chinese]

Author information

  • 1College of Public Health, Zhengzhou University, Zhengzhou 450001, China.

Abstract

OBJECTIVE:

To examine the effect of exogenous zinc on enzymatic activity of tumor suppressor PTEN and underlying mechanisms.

METHODS:

Human airway epithelial cells (BEAS-2B) were employed as the in vitro model. Enzymatic activity of PTEN was measured using malachite green colorimetric assay and immunoblotting. Moreover, the activity of casein kinase-2 (CK-2), the upstream kinase of PTEN, was determined.

RESULTS:

Exposure of BEAS-2B cells to Zn2+ inhibited phosphatase activity of PTEN, as well as PTEN phosphorylation, indicative of PTEN activity (P < 0.05). Under the same experimental condition, Zn2+ stimulation resulted in significant reduction of CK-2 activity (P < 0.05). In addition, CK-2 was shown to induce PTEN phosphorylation (P < 0.05).

CONCLUSION:

These results suggested that Zn2+ exposure might inhibit PTEN phosphatase activity through inhibition of CK-2 activity in human airway epithelial cells.

PMID:
24024362
[PubMed - in process]
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