Format

Send to

Choose Destination
See comment in PubMed Commons below
Int J Exp Pathol. 2013 Oct;94(5):329-35. doi: 10.1111/iep.12040.

Evaluation of autologous blood clot subsegmental pulmonary thromboembolism in minimally invasive experimental canine model.

Author information

  • 1BK21 Basic & Diagnostic Veterinary Specialist Program for Animal Diseases, Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, South Korea.

Abstract

The incidence and significance of subsegmental pulmonary (SSP) thromboembolism is currently under investigation. We aimed to evaluate the clinical and diagnostic features of SSP thromboembolism in an experimental canine model. Obstruction of pulmonary arterial branches was induced in three beagle dogs by intravenous injection of a barium-coated autologous blood clot (size, approximately 1.7 × 5 mm). The clinical signs, haemodynamic changes (blood pressure, electrocardiogram, echocardiography), coagulation (aPTT, PT, FDPs and D-dimer test) and cytokine variations (TNF-a, IL-4, IL-6, and IL-10) were evaluated over a 24-hour period. Multidetector computed tomography (MDCT) with contrast was conducted to evaluate the pulmonary obstruction, and histopathological confirmation was performed. Pulmonary artery pressure gradient (PAPG) was increased 12 h after the autologous blood clot injection (14.2 ± 2.8 mmHg to 23.6 ± 1.7 mmHg, P = 0.003) and normalized 24 h later (P < 0.01). Infused radiopaque clots were confirmed with MDCT and histopathological examination. Pulmonary parenchymal changes such as arterial dilation and inflammatory reactions were also confirmed in histopathological examinations and were barely observable in MDCT. Usually small emboli are not easily detected through CT imaging, and the clinical relevance of these emboli is controversial. In this experimental study, we made radiopaque small emboli and induced SSP thromboembolism. Thus, we infer that obstruction of the small segmental and subsegmental pulmonary arteries does result in a pulmonary thromboembolism (PTE) and PTE-related pulmonary parenchymal changes which could be clinically significant.

© 2013 The Authors. International Journal of Experimental Pathology © 2013 International Journal of Experimental Pathology.

KEYWORDS:

autologous blood clot; experimental canine model; subsegmental pulmonary thromboembolism

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Write to the Help Desk