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Chembiochem. 2013 Nov 4;14(16):2100-5. doi: 10.1002/cbic.201300400. Epub 2013 Sep 9.

Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings.

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  • 1Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037 (USA).


Unnatural amino acids (UAAs) containing conjugated ring systems are of interest for their optical properties. Until now, such bulky and planar UAAs could not be incorporated into proteins using the pyrrolysyl tRNA/synthetase shuttling system. Using the "small-intelligent" approach to construct a highly diverse library, we evolved novel synthetases specific for two such UAAs and incorporated them into proteins in E. coli and mammalian cells.

Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


directed evolution; genetic code expansion; pyrrolysine; small-intelligent library; unnatural amino acids

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