Format

Send to:

Choose Destination
See comment in PubMed Commons below
Burns. 2014 Feb;40(1):61-6. doi: 10.1016/j.burns.2013.05.006. Epub 2013 Sep 7.

Clinical application and viability of cryopreserved cadaveric skin allografts in severe burn: a retrospective analysis.

Author information

  • 1Skin Culture Laboratory, Victorian Adult Burns Service and Department of Surgery, Monash University, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: H.Cleland@alfred.org.au.
  • 2Victorian Adult Burns Service and School of Public Health and Preventive Medicine, Monash University, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: J.Wasiak@alfred.org.au.
  • 3Skin Culture laboratory, Victorian Adult Burns Service, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: hannah.dobson@gmail.com.
  • 4Skin Culture laboratory, Victorian Adult Burns Service, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: M.Paul@alfred.org.au.
  • 5Skin Culture laboratory, Victorian Adult Burns Service, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: G.Pratt@alfred.org.au.
  • 6School of Public Health and Preventive Medicine, Monash University, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: eldho.paul@monash.edu.
  • 7Donor Tissue Bank of Victoria, 57-83 Kavanagh Street, Southbank, Melbourne, Australia. Electronic address: marisah@vifm.org.
  • 8Skin Culture Laboratory, Victorian Adult Burns Service and Department of Surgery, Monash University, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia. Electronic address: shiva.akbarzadeh@monash.edu.

Abstract

INTRODUCTION:

Cadaveric cutaneous allografts are used in burns surgery both as a temporary bio-dressing and occasionally as definitive management of partial thickness burns. Nonetheless, limitations in the understanding of the biology of these grafts have meant that their role in burns surgery continues to be controversial.

METHODS:

A review of all patients suffering 20% or greater total body surface area (TBSA) burns over an eight year period that received cadaveric allografts were identified. To investigate whether tissue viability plays a role in engraftment success, five samples of cryopreserved cadaveric cutaneous allograft processed at the Donor Tissue Bank of Victoria (DTBV) were submitted to our laboratory for viability analysis using two methods of Trypan Blue Exclusion and tetrazolium salt (MTT) assays.

RESULTS:

During the study period, 36 patients received cadaveric allograft at our institution. The average total burn surface area (TBSA) for this group of patients was 40% and all patients received cadaveric skin as a temporizing measure prior to definitive grafting. Cadaveric allograft was used in complicated cases such as wound contamination, where synthetic dressings had failed. Viability tests showed fewer than 30% viability in processed allografts when compared to fresh skin following the thawing process. However, the skin structure in the frozen allografts was histologically well preserved.

CONCLUSION:

Cryopreserved cutaneous cadaveric allograft has a positive and definite role as an adjunct to conventional dressing and grafting where available, particularly in patients with large TBSA burns. The low viability of cryopreserved specimens processed at DTBV suggests that cell viability in cadaveric allograft may not be essential for its clinical function as a wound dressing or even as permanent dermal substitute.

Copyright © 2013 Elsevier Ltd and ISBI. All rights reserved.

KEYWORDS:

Allograft; Burns; Cadaver; Skin; Viability

PMID:
24018216
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk