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ACS Nano. 2013 Oct 22;7(10):9147-55. doi: 10.1021/nn4037706. Epub 2013 Sep 12.

Fluorescent imaging of single nanoparticles and viruses on a smart phone.

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  • 1Electrical Engineering Department, ‡Bioengineering Department, §California NanoSystems Institute (CNSI), ∥Department of Molecular and Medical Pharmacology, and ⊥Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), California 90095, United States.

Abstract

Optical imaging of nanoscale objects, whether it is based on scattering or fluorescence, is a challenging task due to reduced detection signal-to-noise ratio and contrast at subwavelength dimensions. Here, we report a field-portable fluorescence microscopy platform installed on a smart phone for imaging of individual nanoparticles as well as viruses using a lightweight and compact opto-mechanical attachment to the existing camera module of the cell phone. This hand-held fluorescent imaging device utilizes (i) a compact 450 nm laser diode that creates oblique excitation on the sample plane with an incidence angle of ~75°, (ii) a long-pass thin-film interference filter to reject the scattered excitation light, (iii) an external lens creating 2× optical magnification, and (iv) a translation stage for focus adjustment. We tested the imaging performance of this smart-phone-enabled microscopy platform by detecting isolated 100 nm fluorescent particles as well as individual human cytomegaloviruses that are fluorescently labeled. The size of each detected nano-object on the cell phone platform was validated using scanning electron microscopy images of the same samples. This field-portable fluorescence microscopy attachment to the cell phone, weighing only ~186 g, could be used for specific and sensitive imaging of subwavelength objects including various bacteria and viruses and, therefore, could provide a valuable platform for the practice of nanotechnology in field settings and for conducting viral load measurements and other biomedical tests even in remote and resource-limited environments.

PMID:
24016065
[PubMed - indexed for MEDLINE]
PMCID:
PMC3951925
Free PMC Article
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