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Cloning of cDNA and genomic DNA for human cytochrome P-45011 beta.
Department of Medical Chemistry, Kochi Medical School, Japan.
A full-length cDNA clone encoding steroid 11 beta-hydroxylase (P-45011 beta) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-450(11) beta gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-450(11) beta gene.
PMID: 2401360 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Construction of 3D models of the CYP11B family as a tool to predict ligand binding characteristics.
Roumen L, Sanders MP, Pieterse K, Hilbers PA, Plate R, Custers E, de Gooyer M, Smits JF, Beugels I, Emmen J, et al.
J Comput Aided Mol Des. 2007 Aug; 21(8):455-71. Epub 2007 Jul 24.
[J Comput Aided Mol Des. 2007]
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Mutation T318M in the CYP11B2 gene encoding P450c11AS (aldosterone synthase) causes corticosterone methyl oxidase II deficiency.
Zhang G, Rodriguez H, Fardella CE, Harris DA, Miller WL.
Am J Hum Genet. 1995 Nov; 57(5):1037-43.
[Am J Hum Genet. 1995]
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New nucleotide sequence data on the EMBL File Server.
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Nucleic Acids Res. 1990 Dec 25; 18(24):7473-6.
[Nucleic Acids Res. 1990]
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