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Neuroscience. 2013 Dec 3;253:245-55. doi: 10.1016/j.neuroscience.2013.08.046. Epub 2013 Sep 3.

Regulation of α3-containing GABAA receptors in guinea-pig adrenal medullary cells by adrenal steroids.

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  • 1Department of Cell and Systems Physiology, University of Occupational and Environmental Health School of Medicine, Kitakyushu 807-8555, Japan. Electronic address: minoue@med.uoeh-u.ac.jp.

Abstract

GABA is thought to function as a paracrine factor in adrenal medullary (AM) cells. Thus, we electrophysiologically and immunologically examined the properties of GABAA receptors (GABAARs) in guinea-pig AM cells. Bath application of GABA produced an inward current at -60 mV in a dose-dependent manner with an EC50 of 32.3 μM. This GABA-induced current was enhanced by allopregnanolone at concentrations of 0.01 μM and more. A prior exposure to allopregnanolone resulted in a decrease in an EC50 for GABA in activating GABAARs. The GABA-induced current was suppressed by Zn(2+) in a dose-dependent manner with an IC50 of 18 μM, whereas it was enhanced by 100 μM La(3+). The benzodiazepine analog diazepam was three times more potent than zolpidem in enhancing the GABA current, and it was also augmented by L-838,417, which has no action on α1-containing GABAARs. The GABAAR α3, but not α1, and γ2 subunits were immunologically detected at the cell periphery. The expression of α3 subunits in PC12 cells was enhanced by glucocorticoid activity. The results indicated that GABAARs in guinea-pig AM cells mainly comprise α3, β, and γ2 subunits and are enhanced by allopreganalone and glucocorticoids may play a major role in the expression of α3 subunits.

Copyright © 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

KEYWORDS:

AM; Ab; DHEAS; DMSO; GABA; GABA(A) receptor; GABA(A)R; Hepes; IRs; PAGE; PBS-T; PC12; PVDF; RT-PCR; SDS; adrenal medullary; antibody; chromaffin cell; dehydroepiandrostedione sulfate; dimethyl sulfoxide; glucocorticoid; hydroxyethyl piperazineethanesulfonic acid; immunoreactivities; phosphate-buffered saline with Tween 20; polyacrylamide gel electrophoresis; polyvinylidene difluoride; receptor; reverse transcriptase-polymerase chain reaction; sodium dodecyl sulfate; steroid hormone

PMID:
24012744
[PubMed - indexed for MEDLINE]
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