The utility of Brilliant Cresyl Blue (BCB) staining of mammalian oocytes used for in vitro embryo production (IVP)

Jolanta Opiela; Lucyna Kątska-Książkiewicz

doi:10.1016/j.repbio.2013.07.004

The utility of Brilliant Cresyl Blue (BCB) staining of mammalian oocytes used for in vitro embryo production (IVP)

Reprod Biol. 2013 Sep;13(3):177-83. doi: 10.1016/j.repbio.2013.07.004. Epub 2013 Jul 20.

Authors

Jolanta Opiela¹, Lucyna Kątska-Książkiewicz

Affiliation

¹ National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, Balice/Kraków, Poland. Electronic address: jopiela@izoo.krakow.pl.

PMID: 24011188
DOI: 10.1016/j.repbio.2013.07.004

Abstract

The present article summarizes the results of experiments investigating the Brilliant Cresyl Blue (BCB) staining for selection of immature oocytes before in vitro embryo production or somatic cell nuclear transfer. Developmental competence of oocytes stained with BCB and quality of blastocysts derived from such oocytes as well as the expression of apoptosis-related genes, mitochondrial DNA (mtDNA) replication-related genes and the transcripts encoded by the mitochondrial genome in BCB stained oocytes are discussed.

Keywords: Brilliant Cresyl Blue (BCB); Cattle; Goat; Oocyte; Pig; Sheep.

Copyright © 2013 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Apoptosis / genetics
Cattle
DNA, Mitochondrial / genetics
Female
Fertilization in Vitro
Goats
Nuclear Transfer Techniques
Oocytes / drug effects
Oocytes / metabolism
Oocytes / physiology*
Oxazines*
Reproductive Techniques, Assisted*
Sheep
Staining and Labeling
Swine

Substances

DNA, Mitochondrial
Oxazines
Brilliant Cresyl Blue