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Methods Enzymol. 2013;529:153-60. doi: 10.1016/B978-0-12-418687-3.00012-4.

Preparation of genomic DNA from Saccharomyces cerevisiae.

Author information

  • 1The High Throughput Biology Center and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: jsiege16@jhmi.edu.

Abstract

The ability to isolate genomic DNA rapidly and effectively for analysis by PCR, Southern blotting, or other methods is an essential skill. This protocol provides a fast and efficient method for obtaining genomic DNA from S. cerevisiae.

Copyright © 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Cell harvesting; DNA extraction; PCR; Purification of DNA preparation; Saccharomyces cerevisiae

PMID:
24011043
[PubMed - indexed for MEDLINE]
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