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Methods Enzymol. 2013;529:29-34. doi: 10.1016/B978-0-12-418687-3.00003-3.

Explanatory chapter: introducing exogenous DNA into cells.

Author information

  • 1Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: laurakoontz@gmail.com.

Abstract

The ability to efficiently introduce DNA into cells is essential for many experiments in biology. This is an explanatory chapter providing an overview of the various methods for introducing DNA into bacteria, yeast, and mammalian cells.

Copyright © 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Bacteria transformation; Calcium chloride transformation; Eukaryotic cells; Exogenous DNA; Lithium acetate transformation; Yeast transformation

PMID:
24011034
[PubMed - indexed for MEDLINE]
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